Utilization of the vibrating probe and ion-selective microelectrode techniques to investigate electrophysiological responses to wounding in pea roots

J. M. Hush*, I. A. Newman, R. L. Overall

*Corresponding author for this work

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

This paper examines the ionic composition of wound-induced electrical currents in higher plant tissue, using two non-injurious electrophysiological techniques. By simultaneous recording of K+, H+ , and Ca2+ ion fluxes with extracellular ion-selective microelectrodes, we have determined that a Ca2+ influx (2.4 μA cm-2), a small H+ influx (0.17 μA cm-2) and a large K+ efflux (16 μA cm-2) occur immediately after wounding in roots of Pisum sativum L. var. Greenfeast. Using an extracellular vibrating probe at the wound site, net ion currents of 26 μA cm-2 were measured 5 min after wounding. In a more concentrated bathing medium (1/4 rather than 1/16 strength Hoagland's solution), net ion currents of 59 μA cm-2 were measured, and these would appear to be the largest extracellular currents that have been measured in plants. We made a quantitative comparison of the summed ion fluxes with the net ion currents and this revealed that ion fluxes, in addition to those measured here, occur after wounding.

Original languageEnglish
Pages (from-to)1251-1257
Number of pages7
JournalJournal of Experimental Botany
Volume43
Issue number9
DOIs
Publication statusPublished - Sep 1992
Externally publishedYes

Keywords

  • Calcium
  • Electric current
  • Ion flux
  • Potassium
  • Wounding

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