UV LED excited time-gated luminescence flow cytometry

Concepts and experimental evaluation

Jin Dayong*, Russell Connally, Jim Piper

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

Abstract

This paper presents experimental and theoretical studies of time-gated discrimination of long-lived luminescence (lifetime: 1-2000 μs) labelled target-organisms against non-target autofluorescence background (lifetime: <100 ns) in flow cytometry. A theoretical model of such a TGL flow cytometer is developed which takes account of flow speed, illumination and detection apertures, fluorescence label lifetime, and pulsed illumination and gated detection timing sequences. Ultraviolet LED and channel photomultiplier were found to be practical as pulsed excitation sources and gated detector for TGL flow cytometry. The prototype cytometer was constructed and optimized to operate at 6 k Hz repetition rate of TGL cycles consisting of 100 μs LED excitation and ∼60 μs gated detection. The spatial counting efficiency was evaluated by enumerating 5.5 μm diameter europium microspheres resulting in a counting accuracy approaching 100%.

Original languageEnglish
Title of host publicationSmart Medical and Biomedical Sensor Technology IV
EditorsBrian M. Cullum, J. Chance Carter
Place of PublicationBellingham, WA
PublisherSPIE
Pages1-8
Number of pages8
Volume6380
ISBN (Print)0819464783, 9780819464781
DOIs
Publication statusPublished - 2006
EventSmart Medical and Biomedical Sensor Technology IV - Boston, MA, United States
Duration: 3 Oct 20064 Oct 2006

Other

OtherSmart Medical and Biomedical Sensor Technology IV
CountryUnited States
CityBoston, MA
Period3/10/064/10/06

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