UV LED excited time-gated luminescence flow cytometry: evaluation for rare-event particle counting

Dayong Jin*, Belinda Ferrari, Robert C. Leif, Sean Yang, Lidia M. Vallarino, John Williams, James Piper

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

2 Citations (Scopus)
3 Downloads (Pure)

Abstract

Flow cytometric detection of specific rare-event targets within high-background samples such as water or food are frequently defeated by the extremely large population of non-target background particles. Time-gated detection of long lifetime fluorescence (>10μs) labeled microbial targets has been proven highly efficient in suppressing this non-target autofluorescent (<0.1μs) background. A time-gated luminescence (TGL) flow cytometer using UV LED excitation has demonstrated the successful detection of rare-event particles in high autofluorescence background samples. In this report, high-quality 5μm europium beads were made (homogenous intensity and aggregation free) for a detailed evaluation of the prototype performance. The known number of beads (10±2, 100±20 and 1000±100) were first sorted by a conventional flow cytometry sorter, and spiked into an environmental water concentrate (1 ml; containing >10 million non-target particles). The recovery rate for counting these very-rare-event particles using the TGL flow cytometer was then found to be 100%±20% between bead concentrations evaluated.

Original languageEnglish
Title of host publicationImaging, manipulation, and analysis of biomolecules, cells, and tissues VI
Subtitle of host publication21-23 January 2008, San Jose, California, USA
EditorsDaniel L. Farkas, Dan V. Nicolau, Robert C. Leif
Place of PublicationBellingham, Washington
PublisherSPIE
Pages68590O-1-68590O-11
Number of pages11
ISBN (Print)9780819470348
DOIs
Publication statusPublished - 2008
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI - San Jose, CA, United States
Duration: 21 Jan 200823 Jan 2008

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume6859
ISSN (Print)1605-7422

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI
CountryUnited States
CitySan Jose, CA
Period21/01/0823/01/08

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