@inproceedings{247f7e368d374ae5b3dbca2916787d0f,
title = "UV LED excited time-gated luminescence flow cytometry: evaluation for rare-event particle counting",
abstract = "Flow cytometric detection of specific rare-event targets within high-background samples such as water or food are frequently defeated by the extremely large population of non-target background particles. Time-gated detection of long lifetime fluorescence (>10μs) labeled microbial targets has been proven highly efficient in suppressing this non-target autofluorescent (<0.1μs) background. A time-gated luminescence (TGL) flow cytometer using UV LED excitation has demonstrated the successful detection of rare-event particles in high autofluorescence background samples. In this report, high-quality 5μm europium beads were made (homogenous intensity and aggregation free) for a detailed evaluation of the prototype performance. The known number of beads (10±2, 100±20 and 1000±100) were first sorted by a conventional flow cytometry sorter, and spiked into an environmental water concentrate (1 ml; containing >10 million non-target particles). The recovery rate for counting these very-rare-event particles using the TGL flow cytometer was then found to be 100%±20% between bead concentrations evaluated.",
author = "Dayong Jin and Belinda Ferrari and Leif, {Robert C.} and Sean Yang and Vallarino, {Lidia M.} and John Williams and James Piper",
year = "2008",
doi = "10.1117/12.762077",
language = "English",
isbn = "9780819470348",
series = "Proceedings of SPIE",
publisher = "SPIE",
pages = "68590O--1--68590O--11",
editor = "Farkas, {Daniel L.} and Nicolau, {Dan V.} and Leif, {Robert C.}",
booktitle = "Imaging, manipulation, and analysis of biomolecules, cells, and tissues VI",
address = "United States",
note = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VI ; Conference date: 21-01-2008 Through 23-01-2008",
}