Abstract
The invention provides a method for producing a replicase comprising EF-Ts, EF-Tu and β polypeptide subunits, the method comprising expressing in a suitable host cell the EF-Ts and EF-Tu subunits, followed by expressing the β subunit, wherein the EF-Ts and EF-Tu subunits are operably linked to a first promoter and the β subunit is operably linked to a second promoter, and wherein the first and second promoters are differentially induced.
Original language | English |
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Patent number | WO2008106739 |
IPC | C12N 15/52 (2006.01) |
Priority date | 6/03/07 |
Publication status | Published - 12 Sept 2008 |