Versatile application of fluorescent quantum dot labels in super-resolution fluorescence microscopy

Xusan Yang, Karl Zhanghao, Hening Wang, Yujia Liu, Fan Wang, Xu Zhang, Kebin Shi, Juntao Gao*, Dayong Jin, Peng Xi

*Corresponding author for this work

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Quantum dots (QDs) are well known as bright and photostable inorganic fluorescent probes for microscopy imaging, with many attractive features superior to those found in organic dyes. However, their broadband excitation spectrum and emission blinking property have limited the applicability of QDs in modern super-resolution microscopy techniques. In this work, we systematically investigate practical approaches to overcoming these drawbacks and provide examples of their use across many commercially available super-resolution microscopy systems now accessible to biologists, with examples across the major super-resolution techniques. This work further maps out how QDs can be further engineered to facilitate their applications in the respective super-resolution microscopy techniques.

Original languageEnglish
Pages (from-to)1611-1618
Number of pages8
JournalACS Photonics
Volume3
Issue number9
DOIs
Publication statusPublished - 21 Sep 2016

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    ARC CoE Nanoscale BioPhotonics (CNBP) (RAAP)

    Piper, J., Goldys, E., Packer, N. & Jin, D.

    20/06/14 → …

    Project: Research

    Cite this

    Yang, X., Zhanghao, K., Wang, H., Liu, Y., Wang, F., Zhang, X., ... Xi, P. (2016). Versatile application of fluorescent quantum dot labels in super-resolution fluorescence microscopy. ACS Photonics, 3(9), 1611-1618. https://doi.org/10.1021/acsphotonics.6b00178