A Versatile upconversion surface evaluation platform for bio-nano surface selection for the nervous system

Libing Fu, Marco Morsch, Bingyang Shi, Guoying Wang, Albert Lee, Rowan Radford, Yiqing Lu, Dayong Jin, Roger Chung

Research output: Contribution to journalArticleResearchpeer-review

Abstract

There is considerable interest in developing diagnostic nanotools for early detection and delivery of various therapeutic agents for treatment of neurodegenerative diseases. However, a key challenge remains in the selection of suitable surfaces to overcome the nano-bio interface issue, namely that many nanoparticle surfaces demonstrate instability when administered into biological environments and show substantial cytotoxicity to the central nervous system. In this study, we fabricated an evaluation platform for bio-nano surface selection based on the combination of upconversion nanoparticles (UCNPs), cultured neural cells and zebra fish, and systemically demonstrated how it can evaluate the suitability of nanoparticle surfaces for applications in the central nervous system. Firstly, we fabricated highly lanthanide-doped UCNPs, which generate the strongest tissue penetrable emission at 800 nm. We then functionalized these UCNPs with four popular surfaces for evaluation. Next, we systematically evaluated the spectral emission properties, biophysical stability, cytotoxicity and cell uptake capability of these surface-functionalized UCNPs in biological solutions or with cultured NSC-34 cells. Through these studies, PEG-COOH proved to be the superior surface modification. Accordingly, we further confirmed the bioavailability of unmodified and surface modified UCNPs in the spinal cord of living zebrafish. As predicted, PEG-UCNPs displayed excellent dispersal and uptake into spinal motor neurons in living zebrafish. Collectively, this study developed a versatile upconversion platform for systematic evaluation of nanoparticle surfaces, which can provide valuable information via systemic surface evaluation in vitro and in vivo for future construction of multifunctional nanosystems for theranostic applications in neurodegenerative diseases.

LanguageEnglish
Pages13683-13692
Number of pages10
JournalNanoscale
Volume9
Issue number36
DOIs
Publication statusPublished - 17 Aug 2017

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Neurology
Nanoparticles
Neurodegenerative diseases
Cytotoxicity
Polyethylene glycols
Nanosystems
Lanthanoid Series Elements
Rare earth elements
Fish
Neurons
Surface treatment
Tissue

Cite this

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title = "A Versatile upconversion surface evaluation platform for bio-nano surface selection for the nervous system",
abstract = "There is considerable interest in developing diagnostic nanotools for early detection and delivery of various therapeutic agents for treatment of neurodegenerative diseases. However, a key challenge remains in the selection of suitable surfaces to overcome the nano-bio interface issue, namely that many nanoparticle surfaces demonstrate instability when administered into biological environments and show substantial cytotoxicity to the central nervous system. In this study, we fabricated an evaluation platform for bio-nano surface selection based on the combination of upconversion nanoparticles (UCNPs), cultured neural cells and zebra fish, and systemically demonstrated how it can evaluate the suitability of nanoparticle surfaces for applications in the central nervous system. Firstly, we fabricated highly lanthanide-doped UCNPs, which generate the strongest tissue penetrable emission at 800 nm. We then functionalized these UCNPs with four popular surfaces for evaluation. Next, we systematically evaluated the spectral emission properties, biophysical stability, cytotoxicity and cell uptake capability of these surface-functionalized UCNPs in biological solutions or with cultured NSC-34 cells. Through these studies, PEG-COOH proved to be the superior surface modification. Accordingly, we further confirmed the bioavailability of unmodified and surface modified UCNPs in the spinal cord of living zebrafish. As predicted, PEG-UCNPs displayed excellent dispersal and uptake into spinal motor neurons in living zebrafish. Collectively, this study developed a versatile upconversion platform for systematic evaluation of nanoparticle surfaces, which can provide valuable information via systemic surface evaluation in vitro and in vivo for future construction of multifunctional nanosystems for theranostic applications in neurodegenerative diseases.",
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A Versatile upconversion surface evaluation platform for bio-nano surface selection for the nervous system. / Fu, Libing; Morsch, Marco; Shi, Bingyang; Wang, Guoying; Lee, Albert; Radford, Rowan; Lu, Yiqing; Jin, Dayong; Chung, Roger.

In: Nanoscale, Vol. 9, No. 36, 17.08.2017, p. 13683-13692.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - A Versatile upconversion surface evaluation platform for bio-nano surface selection for the nervous system

AU - Fu,Libing

AU - Morsch,Marco

AU - Shi,Bingyang

AU - Wang,Guoying

AU - Lee,Albert

AU - Radford,Rowan

AU - Lu,Yiqing

AU - Jin,Dayong

AU - Chung,Roger

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