Virome composition in marine fish revealed by meta-transcriptomics

Jemma L. Geoghegan*, Francesca Di Giallonardo, Michelle Wille, Ayda Susana Ortiz-Baez, Vincenzo A. Costa, Timothy Ghaly, Jonathon C. O. Mifsud, Olivia M. H. Turnbull, David R. Bellwood, Jane E. Williamson, Edward C. Holmes

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)
    9 Downloads (Pure)

    Abstract

    Revealing the determinants of virome composition is central to placing disease emergence in a broader evolutionary context. Fish are the most species-rich group of vertebrates and so provide an ideal model system to study the factors that shape virome compositions and their evolution. We characterized the viromes of nineteen wild-caught species of marine fish using total RNA sequencing (meta-transcriptomics) combined with analyses of sequence and protein structural homology to identify divergent viruses that often evade characterization. From this, we identified twenty-five new vertebrate-associated viruses and a further twenty-two viruses likely associated with fish diet or their microbiomes. The vertebrate-associated viruses identified here included the first fish virus in the Matonaviridae (single-strand, positive-sense RNA virus). Other viruses fell within the Astroviridae, Picornaviridae, Arenaviridae, Reoviridae, Hepadnaviridae, Paramyxoviridae, Rhabdoviridae, Hantaviridae, Filoviridae, and Flaviviridae, and were sometimes phylogenetically distinct from known fish viruses. We also show how key metrics of virome composition—viral richness, abundance, and diversity—can be analysed along with host ecological and biological factors as a means to understand virus ecology. Accordingly, these data suggest that that the vertebrate-associated viromes of the fish sampled here are predominantly shaped by the phylogenetic history (i.e. taxonomic order) of their hosts, along with several biological factors including water temperature, habitat depth, community diversity and swimming behaviour. No such correlations were found for viruses associated with porifera, molluscs, arthropods, fungi, and algae, that are unlikely to replicate in fish hosts. Overall, these data indicate that fish harbour particularly large and complex viromes and the vast majority of fish viromes are undescribed.

    Original languageEnglish
    Article numberveab005
    Pages (from-to)1-11
    Number of pages11
    JournalVirus Evolution
    Volume7
    Issue number1
    DOIs
    Publication statusPublished - Jan 2021

    Bibliographical note

    Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

    Erratum: Jemma L Geoghegan, Francesca Di Giallonardo, Michelle Wille, Ayda Susana Ortiz-Baez, Vincenzo A Costa, Timothy Ghaly, Jonathon C O Mifsud, Olivia M H Turnbull, David R Bellwood, Jane E Williamson, Edward C Holmes, Erratum: Virome composition in marine fish revealed by meta-transcriptomics, Virus Evolution, Volume 7, Issue 1, January 2021, veab035, https://doi.org/10.1093/ve/veab035

    Keywords

    • fish
    • virome
    • virus evolution
    • metagenomics
    • host-jumping

    Fingerprint

    Dive into the research topics of 'Virome composition in marine fish revealed by meta-transcriptomics'. Together they form a unique fingerprint.

    Cite this