Viscoelastic separation of drug-treated E. coli by shape

Tianlong Zhang; Yaxiaer Yalikun; Kazunori Okano; Yo Tanaka; David W. Inglis; Yoichiroh Hosokawa; Ming Li

Viscoelastic separation of drug-treated E. coli by shape

Tianlong Zhang, Yaxiaer Yalikun^*, Kazunori Okano, Yo Tanaka, David W. Inglis, Yoichiroh Hosokawa, Ming Li^*

^*Corresponding author for this work

School of Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

Abstract

Here, we achieve viscoelastic separation of drug-treated Escherichia coli (E. coli) by shape. Since shape is critical for modulating biological functions of E. coli, the ability to prepare homogeneous E. coli populations adopting uniform shape or sort bacterial sub-population based on their shape has significant implications for a broad range of biological, biomedical and environmental applications. A proportion of E. coli treated with 1 μg/mL of the antibiotic mecillinam demonstrate changes in shape from rod to sphere, and the heterogeneous E. coli populations after drug treatment with various aspect ratios (ARs) ranging from 1.0 to 5.5 were used for experiment. We demonstrate that spherical E. coli (ARİ1.5) are mainly directed toward the middle outlet, while rod-shaped E. coli with a higher AR (AR>1.5) are driven to the side outlets.

Original language	English
Title of host publication	MicroTAS 2022
Subtitle of host publication	The 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Place of Publication	California
Publisher	Chemical and Biological Microsystems Society
Pages	929-930
Number of pages	2
ISBN (Electronic)	9781733419048
Publication status	Published - 2022
Event	26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 - Hybrid, Hangzhou, China Duration: 23 Oct 2022 → 27 Oct 2022

Publication series

Name
ISSN (Electronic)	1556-5904

Conference

Conference	26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022
Country/Territory	China
City	Hybrid, Hangzhou
Period	23/10/22 → 27/10/22

Keywords

Viscoelastic Separation
E. coli
Shape
Antibiotic Treatment
Microfluidics

Cite this

@inproceedings{23e0ce4ec0f646e899a3b63ddfe872ae,

title = "Viscoelastic separation of drug-treated E. coli by shape",

abstract = "Here, we achieve viscoelastic separation of drug-treated Escherichia coli (E. coli) by shape. Since shape is critical for modulating biological functions of E. coli, the ability to prepare homogeneous E. coli populations adopting uniform shape or sort bacterial sub-population based on their shape has significant implications for a broad range of biological, biomedical and environmental applications. A proportion of E. coli treated with 1 μg/mL of the antibiotic mecillinam demonstrate changes in shape from rod to sphere, and the heterogeneous E. coli populations after drug treatment with various aspect ratios (ARs) ranging from 1.0 to 5.5 were used for experiment. We demonstrate that spherical E. coli (ARİ1.5) are mainly directed toward the middle outlet, while rod-shaped E. coli with a higher AR (AR>1.5) are driven to the side outlets.",

keywords = "Viscoelastic Separation, E. coli, Shape, Antibiotic Treatment, Microfluidics",

author = "Tianlong Zhang and Yaxiaer Yalikun and Kazunori Okano and Yo Tanaka and Inglis, {David W.} and Yoichiroh Hosokawa and Ming Li",

year = "2022",

language = "English",

publisher = "Chemical and Biological Microsystems Society",

pages = "929--930",

booktitle = "MicroTAS 2022",

note = "26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022 ; Conference date: 23-10-2022 Through 27-10-2022",

}

Zhang, T, Yalikun, Y, Okano, K, Tanaka, Y, Inglis, DW, Hosokawa, Y & Li, M 2022, Viscoelastic separation of drug-treated E. coli by shape. in MicroTAS 2022: The 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, California, pp. 929-930, 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022, Hybrid, Hangzhou, China, 23/10/22.

Viscoelastic separation of drug-treated E. coli by shape. / Zhang, Tianlong; Yalikun, Yaxiaer; Okano, Kazunori et al.
MicroTAS 2022: The 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. California: Chemical and Biological Microsystems Society, 2022. p. 929-930.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

TY - GEN

T1 - Viscoelastic separation of drug-treated E. coli by shape

AU - Zhang, Tianlong

AU - Yalikun, Yaxiaer

AU - Okano, Kazunori

AU - Tanaka, Yo

AU - Inglis, David W.

AU - Hosokawa, Yoichiroh

AU - Li, Ming

PY - 2022

Y1 - 2022

N2 - Here, we achieve viscoelastic separation of drug-treated Escherichia coli (E. coli) by shape. Since shape is critical for modulating biological functions of E. coli, the ability to prepare homogeneous E. coli populations adopting uniform shape or sort bacterial sub-population based on their shape has significant implications for a broad range of biological, biomedical and environmental applications. A proportion of E. coli treated with 1 μg/mL of the antibiotic mecillinam demonstrate changes in shape from rod to sphere, and the heterogeneous E. coli populations after drug treatment with various aspect ratios (ARs) ranging from 1.0 to 5.5 were used for experiment. We demonstrate that spherical E. coli (ARİ1.5) are mainly directed toward the middle outlet, while rod-shaped E. coli with a higher AR (AR>1.5) are driven to the side outlets.

AB - Here, we achieve viscoelastic separation of drug-treated Escherichia coli (E. coli) by shape. Since shape is critical for modulating biological functions of E. coli, the ability to prepare homogeneous E. coli populations adopting uniform shape or sort bacterial sub-population based on their shape has significant implications for a broad range of biological, biomedical and environmental applications. A proportion of E. coli treated with 1 μg/mL of the antibiotic mecillinam demonstrate changes in shape from rod to sphere, and the heterogeneous E. coli populations after drug treatment with various aspect ratios (ARs) ranging from 1.0 to 5.5 were used for experiment. We demonstrate that spherical E. coli (ARİ1.5) are mainly directed toward the middle outlet, while rod-shaped E. coli with a higher AR (AR>1.5) are driven to the side outlets.

KW - Viscoelastic Separation

KW - E. coli

KW - Shape

KW - Antibiotic Treatment

KW - Microfluidics

UR - http://www.scopus.com/inward/record.url?scp=85175709591&partnerID=8YFLogxK

UR - http://purl.org/au-research/grants/arc/DP200102269

UR - https://cbmsociety.org/conference/proceedings_archive.html

M3 - Conference proceeding contribution

AN - SCOPUS:85175709591

SP - 929

EP - 930

BT - MicroTAS 2022

PB - Chemical and Biological Microsystems Society

CY - California

T2 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2022

Y2 - 23 October 2022 through 27 October 2022

ER -

Viscoelastic separation of drug-treated E. coli by shape

Abstract

Publication series

Conference

Keywords

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Cell-Sort MultiTool: a Novel Platform for Single-cell Bacteria Analysis

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Viscoelastic separation of drug-treated E. coli by shape

Abstract

Publication series

Conference

Keywords

Other files and links

Fingerprint

Projects

Cell-Sort MultiTool: a Novel Platform for Single-cell Bacteria Analysis

Cite this