Visual evoked potential recording in a rat model of experimental optic nerve demyelination

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The visual evoked potential (VEP) recording is widely used in clinical practice to assess the severity of optic neuritis in its acute phase, and to monitor the disease course in the follow-up period. Changes in the VEP parameters closely correlate with pathological damage in the optic nerve. This protocol provides a detailed description about the rodent model of optic nerve microinjection, in which a partial demyelination lesion is produced in the optic nerve. VEP recording techniques are also discussed. Using skull implanted electrodes, we are able to acquire reproducible intra-session and between-session VEP traces. VEPs can be recorded on individual animals over a period of time to assess the functional changes in the optic nerve longitudinally. The optic nerve demyelination model, in conjunction with the VEP recording protocol, provides a tool to investigate the disease processes associated with demyelination and remyelination, and can potentially be employed to evaluate the effects of new remyelinating drugs or neuroprotective therapies.

LanguageEnglish
Article numbere52934
Pages1-5
Number of pages5
JournalJournal of Visualized Experiments
Issue number101
DOIs
Publication statusPublished - 29 Jul 2015

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Visual Evoked Potentials
Bioelectric potentials
Demyelinating Diseases
Optic Nerve
Rats
Optics
Theoretical Models
Optic Neuritis
Implanted Electrodes
Microinjections
Neuroprotective Agents
Skull
Rodentia
Animals
Electrodes
Pharmaceutical Preparations

Cite this

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title = "Visual evoked potential recording in a rat model of experimental optic nerve demyelination",
abstract = "The visual evoked potential (VEP) recording is widely used in clinical practice to assess the severity of optic neuritis in its acute phase, and to monitor the disease course in the follow-up period. Changes in the VEP parameters closely correlate with pathological damage in the optic nerve. This protocol provides a detailed description about the rodent model of optic nerve microinjection, in which a partial demyelination lesion is produced in the optic nerve. VEP recording techniques are also discussed. Using skull implanted electrodes, we are able to acquire reproducible intra-session and between-session VEP traces. VEPs can be recorded on individual animals over a period of time to assess the functional changes in the optic nerve longitudinally. The optic nerve demyelination model, in conjunction with the VEP recording protocol, provides a tool to investigate the disease processes associated with demyelination and remyelination, and can potentially be employed to evaluate the effects of new remyelinating drugs or neuroprotective therapies.",
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Visual evoked potential recording in a rat model of experimental optic nerve demyelination. / You, Yuyi; Gupta, Vivek K.; Chitranshi, Nitin; Reedman, Brittany; Klistorner, Alexander; Graham, Stuart L.

In: Journal of Visualized Experiments, No. 101, e52934, 29.07.2015, p. 1-5.

Research output: Contribution to journalArticleResearchpeer-review

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