Abstract
The encapsulation of fat soluble vitamins in nanoliposomes seems to be an effective method for protecting them from light, oxygen and chemical degradation. In this study, nanoliposomes containing Vitamin A palmitate were prepared from different concentrations of lecithin-cholesterol (60:0, 50:10, 40:20 and 30:30 mg) by thin-film hydration-sonication method. Fourier transform infrared spectra (FTIR) were utilized to study the possible bioactive-lipid complex formation and the results indicated that the complex between Vitamin A and liposomes were formed by physical interaction. Particle size, morphology, encapsulation efficiency and physical stability tests were carried out to determine the physicochemical properties of the resulted Vitamin A-bearing liposomes. The size of particles were in the range of 76-115 nm and the particle size distributions were monomodular (span=0.6-0.88). The results showed that using the highest cholesterol concentration for preparing of liposomes containing Vitamin A palmitate induces lower encapsulation efficiency and 50/10 mg lecithin-cholesterol concentration was used for preparation of optimum formulation of Vitamin A palmitate-loaded nanoliposomes with mean size of about 76 nm and monomodular size distribution (span=0.74) and the encapsulation efficiency was 15.8%.
Original language | English |
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Pages (from-to) | 49-55 |
Number of pages | 7 |
Journal | Food Bioscience |
Volume | 13 |
DOIs | |
Publication status | Published - 1 Mar 2016 |
Externally published | Yes |
Keywords
- Encapsulation
- FTIR
- Nanoliposome
- Storage stability
- Vitamin A palmitate