The encapsulation of fat soluble vitamins in nanoliposomes seems to be an effective method for protecting them from light, oxygen and chemical degradation. In this study, nanoliposomes containing Vitamin A palmitate were prepared from different concentrations of lecithin-cholesterol (60:0, 50:10, 40:20 and 30:30 mg) by thin-film hydration-sonication method. Fourier transform infrared spectra (FTIR) were utilized to study the possible bioactive-lipid complex formation and the results indicated that the complex between Vitamin A and liposomes were formed by physical interaction. Particle size, morphology, encapsulation efficiency and physical stability tests were carried out to determine the physicochemical properties of the resulted Vitamin A-bearing liposomes. The size of particles were in the range of 76-115 nm and the particle size distributions were monomodular (span=0.6-0.88). The results showed that using the highest cholesterol concentration for preparing of liposomes containing Vitamin A palmitate induces lower encapsulation efficiency and 50/10 mg lecithin-cholesterol concentration was used for preparation of optimum formulation of Vitamin A palmitate-loaded nanoliposomes with mean size of about 76 nm and monomodular size distribution (span=0.74) and the encapsulation efficiency was 15.8%.
- Storage stability
- Vitamin A palmitate