Wastewater, wheat and table wipes: adventures in culture-independent microbiology

Jacob E. Munro; Deborah J. Rich; Simon Dingsdag; Nicholas V. Coleman

doi:10.1071/MA14061

Wastewater, wheat and table wipes: adventures in culture-independent microbiology

Jacob E. Munro, Deborah J. Rich, Simon Dingsdag, Nicholas V. Coleman^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The sequencing of ribosomal RNA and DNA (rRNA/rDNA) from environmental samples heralded a new age in microbiology^1-3. The advent of next-generation sequencing supercharged these methods, which now give high-resolution data sets, enabling real insights into microbial diversity and function in complex systems^4-7. Here, three local applications of 16S rDNA pyrosequencing are described, which highlight the usefulness of this approach for addressing practical questions in diverse areas of microbiology. Limitations of the sequence-based approach will also be discussed.

Original language	English
Pages (from-to)	188-191
Number of pages	4
Journal	Microbiology Australia
Volume	35
Issue number	4
Early online date	30 Oct 2014
DOIs	https://doi.org/10.1071/MA14061
Publication status	Published - Nov 2014
Externally published	Yes

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title = "Wastewater, wheat and table wipes: adventures in culture-independent microbiology",

abstract = "The sequencing of ribosomal RNA and DNA (rRNA/rDNA) from environmental samples heralded a new age in microbiology1-3. The advent of next-generation sequencing supercharged these methods, which now give high-resolution data sets, enabling real insights into microbial diversity and function in complex systems4-7. Here, three local applications of 16S rDNA pyrosequencing are described, which highlight the usefulness of this approach for addressing practical questions in diverse areas of microbiology. Limitations of the sequence-based approach will also be discussed.",

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AB - The sequencing of ribosomal RNA and DNA (rRNA/rDNA) from environmental samples heralded a new age in microbiology1-3. The advent of next-generation sequencing supercharged these methods, which now give high-resolution data sets, enabling real insights into microbial diversity and function in complex systems4-7. Here, three local applications of 16S rDNA pyrosequencing are described, which highlight the usefulness of this approach for addressing practical questions in diverse areas of microbiology. Limitations of the sequence-based approach will also be discussed.

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Wastewater, wheat and table wipes: adventures in culture-independent microbiology

Abstract

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