What place for polyacrylamide in proteomics?

B. R. Herbert*, J. L. Harry, N. H. Packer, A. A. Gooley, S. K. Pedersen, K. L. Williams

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

91 Citations (Scopus)


Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) continues to deliver high quality protein resolution and dynamic range for the proteomics researcher. To remain as the preferred method for protein separation and characterization, several key steps need to be implemented to ensure quality sample preparation and speed of analysis. Here, we describe the progress made towards establishing 2D-PAGE as the optimal separation tool for proteomics research.

Original languageEnglish
Pages (from-to)S3-S9
Number of pages7
JournalTrends in Biotechnology
Issue number10 Suppl
Publication statusPublished - Oct 2001
Externally publishedYes


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