Wild populations of Sydney rock oysters differ in their proteomic responses to elevated carbon dioxide

E. L. Thompson, L. Parker, V. Amaral, M. J. Bishop, W. A. O'Connor, D. A. Raftos

Research output: Contribution to journalArticleResearchpeer-review

Abstract

This study tested the proteomic responses of three spatially distinct Sydney rock oyster populations to elevated pCO2. Oysters were collected from environmentally different sites, two chronically affected by acid sulfate soil. Oysters from each of the three populations were exposed to ambient (380μatm) or elevated (856 and 1500μatm) pCO2 for 4 weeks. Subsequent proteomic analysis from haemolymph revealed that (1) there were differences between the proteomes of the three populations after exposure to ambient pCO2, and (2) the different oyster populations mounted significantly different responses to elevated pCO2. Proteins that differed significantly in concentration between pCO2 treatments fell into five broad functional categories: energy metabolism, cellular stress responses, the cytoskeleton, protein synthesis and the extracellular matrix. This is consistent with the hypothesis that environmental stress in oysters leads to a generic response involving increased mitochondrial energy production to maintain cellular homeostasis. Proteins involved in the cytoskeleton and energy metabolism were the most differentially expressed and were seen in all three oyster populations. Differences between populations in their proteomic responses suggested that the local environments from which oysters originate may affect their capacity to respond to ocean acidification.

LanguageEnglish
Pages1964-1972
Number of pages9
JournalMarine and Freshwater Research
Volume67
Issue number12
DOIs
Publication statusPublished - 2016

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Saccostrea glomerata
proteomics
wild population
oysters
carbon dioxide
protein
metabolism
rock
acid sulfate soil
homeostasis
environmental stress
cytoskeleton
energy metabolism
energy
acid sulfate soils
matrix
proteome
extracellular matrix
hemolymph
stress response

Cite this

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Wild populations of Sydney rock oysters differ in their proteomic responses to elevated carbon dioxide. / Thompson, E. L.; Parker, L.; Amaral, V.; Bishop, M. J.; O'Connor, W. A.; Raftos, D. A.

In: Marine and Freshwater Research, Vol. 67, No. 12, 2016, p. 1964-1972.

Research output: Contribution to journalArticleResearchpeer-review

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