Xylanases from families 10 and 11 from an extreme thermophile. Bleaching activity on kraft pulp and evolutionary improvement of XYNB

P. L. Bergquist*, D. D. Morris, M. D. Gibbs, R. A. Reeves

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

Abstract

Genes for xylanases from two different glycosyl hydrolase families from the bacterium Dictyoglomus thermophilum Rt46B.1 have been isolated and over-expressed in bacterial and fungal hosts. XynB has shown greater promise than XynA as a bleach-boosting agent for both pine and eucalypt kraft pulps in ECF and TCF sequences. Because of the limits of natural biodiversity, further development of XynB for increased pH range is being explored by enzyme evolution techniques which combine in vitro mutagenesis and recombination. Simulations using in silico population genetics are also being implemented to explore the available sequence space.

Original languageEnglish
Title of host publicationProceedings of the International Conference on Biotechnology in the Pulp and Paper Industry
Editors Anon
PublisherCPPA
Volume1
Publication statusPublished - 1998
EventProceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3) - Vancouver, Can
Duration: 16 Jun 199819 Jun 1998

Other

OtherProceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3)
CityVancouver, Can
Period16/06/9819/06/98

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