Xylanases from families 10 and 11 from an extreme thermophile. Bleaching activity on kraft pulp and evolutionary improvement of XYNB

P. L. Bergquist; D. D. Morris; M. D. Gibbs; R. A. Reeves

Xylanases from families 10 and 11 from an extreme thermophile. Bleaching activity on kraft pulp and evolutionary improvement of XYNB

P. L. Bergquist^*, D. D. Morris, M. D. Gibbs, R. A. Reeves

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

Abstract

Genes for xylanases from two different glycosyl hydrolase families from the bacterium Dictyoglomus thermophilum Rt46B.1 have been isolated and over-expressed in bacterial and fungal hosts. XynB has shown greater promise than XynA as a bleach-boosting agent for both pine and eucalypt kraft pulps in ECF and TCF sequences. Because of the limits of natural biodiversity, further development of XynB for increased pH range is being explored by enzyme evolution techniques which combine in vitro mutagenesis and recombination. Simulations using in silico population genetics are also being implemented to explore the available sequence space.

Original language	English
Title of host publication	Proceedings of the International Conference on Biotechnology in the Pulp and Paper Industry
Editors	Anon
Publisher	CPPA
Volume	1
Publication status	Published - 1998
Event	Proceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3) - Vancouver, Can Duration: 16 Jun 1998 → 19 Jun 1998

Other

Other	Proceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3)
City	Vancouver, Can
Period	16/06/98 → 19/06/98

Cite this

@inproceedings{231681aa2d1749208f296d102433fe52,

title = "Xylanases from families 10 and 11 from an extreme thermophile. Bleaching activity on kraft pulp and evolutionary improvement of XYNB",

abstract = "Genes for xylanases from two different glycosyl hydrolase families from the bacterium Dictyoglomus thermophilum Rt46B.1 have been isolated and over-expressed in bacterial and fungal hosts. XynB has shown greater promise than XynA as a bleach-boosting agent for both pine and eucalypt kraft pulps in ECF and TCF sequences. Because of the limits of natural biodiversity, further development of XynB for increased pH range is being explored by enzyme evolution techniques which combine in vitro mutagenesis and recombination. Simulations using in silico population genetics are also being implemented to explore the available sequence space.",

author = "Bergquist, {P. L.} and Morris, {D. D.} and Gibbs, {M. D.} and Reeves, {R. A.}",

year = "1998",

language = "English",

volume = "1",

editor = "Anon",

booktitle = "Proceedings of the International Conference on Biotechnology in the Pulp and Paper Industry",

publisher = "CPPA",

note = "Proceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3) ; Conference date: 16-06-1998 Through 19-06-1998",

}

Bergquist, PL, Morris, DD, Gibbs, MD & Reeves, RA 1998, Xylanases from families 10 and 11 from an extreme thermophile. Bleaching activity on kraft pulp and evolutionary improvement of XYNB. in Anon (ed.), Proceedings of the International Conference on Biotechnology in the Pulp and Paper Industry. vol. 1, CPPA, Proceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3), Vancouver, Can, 16/06/98.

Xylanases from families 10 and 11 from an extreme thermophile. Bleaching activity on kraft pulp and evolutionary improvement of XYNB. / Bergquist, P. L.; Morris, D. D.; Gibbs, M. D. et al.

Proceedings of the International Conference on Biotechnology in the Pulp and Paper Industry. ed. / Anon. Vol. 1 CPPA, 1998.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

TY - GEN

T1 - Xylanases from families 10 and 11 from an extreme thermophile. Bleaching activity on kraft pulp and evolutionary improvement of XYNB

AU - Bergquist, P. L.

AU - Morris, D. D.

AU - Gibbs, M. D.

AU - Reeves, R. A.

PY - 1998

Y1 - 1998

N2 - Genes for xylanases from two different glycosyl hydrolase families from the bacterium Dictyoglomus thermophilum Rt46B.1 have been isolated and over-expressed in bacterial and fungal hosts. XynB has shown greater promise than XynA as a bleach-boosting agent for both pine and eucalypt kraft pulps in ECF and TCF sequences. Because of the limits of natural biodiversity, further development of XynB for increased pH range is being explored by enzyme evolution techniques which combine in vitro mutagenesis and recombination. Simulations using in silico population genetics are also being implemented to explore the available sequence space.

AB - Genes for xylanases from two different glycosyl hydrolase families from the bacterium Dictyoglomus thermophilum Rt46B.1 have been isolated and over-expressed in bacterial and fungal hosts. XynB has shown greater promise than XynA as a bleach-boosting agent for both pine and eucalypt kraft pulps in ECF and TCF sequences. Because of the limits of natural biodiversity, further development of XynB for increased pH range is being explored by enzyme evolution techniques which combine in vitro mutagenesis and recombination. Simulations using in silico population genetics are also being implemented to explore the available sequence space.

UR - http://www.scopus.com/inward/record.url?scp=0031654426&partnerID=8YFLogxK

M3 - Conference proceeding contribution

AN - SCOPUS:0031654426

VL - 1

BT - Proceedings of the International Conference on Biotechnology in the Pulp and Paper Industry

A2 - Anon, null

PB - CPPA

T2 - Proceedings of the 1998 7th International Conference on Biotechnology in the Pulp and Paper Industry. Part 1 (of 3)

Y2 - 16 June 1998 through 19 June 1998

ER -

Xylanases from families 10 and 11 from an extreme thermophile. Bleaching activity on kraft pulp and evolutionary improvement of XYNB

Abstract

Other

Other files and links

Fingerprint

Cite this