Y chromosome missing protein, TBL1Y, may play an important role in cardiac differentiation

Anna Meyfour, Hassan Ansari, Sara Pahlavan, Shahab Mirshahvaladi, Mostafa Rezaei-Tavirani, Hamid Gourabi, Hossein Baharvand*, Ghasem Hosseini Salekdeh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Despite evidence for sex-specific cardiovascular physiology and pathophysiology, the biological basis for this dimorphism remains to be explored. Apart from hormonal factors, gender-related characteristics may reside in the function of sex chromosomes during cardiac development. In this study, we investigated the differential expression of the male-specific region of the Y chromosome (MSY) genes and their X counterparts during cardiac differentiation of human embryonic stem cells (hESC). We observed alterations in mRNA and protein levels of TBL1Y, PCDH11Y, ZFY, KDM5D, USP9Y, RPS4Y1, DDX3Y, PRY, XKRY, BCORP1, RBMY, HSFY, and UTY, which accompanied changes in intracellular localization. Of them, the abundance of a Y chromosome missing protein, TBL1Y, showed a significant increase during differentiation while the expression level of its X counterpart decreased. Consistently, reducing TBL1Y cellular level using siRNA approach influenced cardiac differentiation by reducing its efficacy as well as increasing the probability of impaired contractions. TBL1Y knockdown may have negatively impacted cardiogenesis by CtBP stabilization. Furthermore, we presented compelling experimental evidence to distinguish TBL1Y from TBL1X, its highly similar X chromosome homologue, and proposed reclassification of TBL1Y as "found missing protein" (PE1). Our results demonstrated that MSY proteins may play an important role in cardiac development.

Original languageEnglish
Pages (from-to)4391-4402
Number of pages12
JournalJournal of Proteome Research
Issue number12
Publication statusPublished - 1 Dec 2017
Externally publishedYes


  • Chromosome-Centric Human Proteome Project (C-HPP)
  • Y chromosome
  • human embryonic stem cell
  • cardiac differentiation
  • TBL1Y


Dive into the research topics of 'Y chromosome missing protein, TBL1Y, may play an important role in cardiac differentiation'. Together they form a unique fingerprint.

Cite this