Yeast differentiation using histone promoter sequences

P Bell

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Aims: To evaluate a new platform for yeast differentiation based on histone promoter regions. Methods and Results: The histone gene amino acid sequences of a wide phylogenetic range of organisms were aligned, and primers designed that were capable of amplifying the divergent promoters of the H3-H4 and H2a-H2b loci from yeast. Analysis indicated that the promoter regions were variable in length between species and represented rapidly changing sequences flanked by highly conserved sequences. Conclusions: The histone promoter regions in yeast provide an excellent locus for the rapid and accurate identification of yeast species. Significance and Impact of the Study: This study describes an alternative platform to the ribosomal internal transcribed spacer (ITS) sequences for the identification of yeast species.
Original languageEnglish
Pages (from-to)388-392
Number of pages5
JournalLetters in Applied Microbiology
Volume38
Issue number5
DOIs
Publication statusPublished - 2004

Keywords

  • Histone
  • ITS
  • Nucleosome
  • Promoter
  • Speciation

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