Yeast differentiation using histone promoter sequences

P Bell

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    Aims: To evaluate a new platform for yeast differentiation based on histone promoter regions. Methods and Results: The histone gene amino acid sequences of a wide phylogenetic range of organisms were aligned, and primers designed that were capable of amplifying the divergent promoters of the H3-H4 and H2a-H2b loci from yeast. Analysis indicated that the promoter regions were variable in length between species and represented rapidly changing sequences flanked by highly conserved sequences. Conclusions: The histone promoter regions in yeast provide an excellent locus for the rapid and accurate identification of yeast species. Significance and Impact of the Study: This study describes an alternative platform to the ribosomal internal transcribed spacer (ITS) sequences for the identification of yeast species.
    Original languageEnglish
    Pages (from-to)388-392
    Number of pages5
    JournalLetters in Applied Microbiology
    Volume38
    Issue number5
    DOIs
    Publication statusPublished - 2004

    Keywords

    • Histone
    • ITS
    • Nucleosome
    • Promoter
    • Speciation

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