Zika viral proteome analysis reveals an epitope cluster within NS3 helicase as a potential vaccine candidate: an in silico study

Md Tangigul Haque, Md Nur Ahad Shah, Shatabdi Paul, Md Kawsar Khan, Payal Barua*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    2 Downloads (Pure)

    Abstract

    Zika virus (ZIKV), a mosquito-borne flavivirus, is now an emerging global public health concern. Currently, the pathogenicity, genetic diversity and the consequences of ZIKV infection are little known and a protective vaccine against ZIKV is urgently needed. In this study, we have taken an immunoinformatics approach to predict epitope cluster regions in the whole proteome (3423 amino acids) of ZIKV. We used a range of bioinformatics algorithms to determine epitopes of CD8+ cytotoxic T-cells (CTL), CD4+ helper T-cells (THL) and B cells. We have predicted an epitope cluster of 23 contiguous amino acids (region 1989–2011, WLEARMLLDNIYLQDGLIASLYR) residing on the NS3 helicase protein in the ZIKV proteome. This epitope cluster contains fourteen CD4+ (THL) epitopes and six CD8+ (CTL) epitopes. Finally, we have validated the epitopes by analysing their binding efficiency (binding energy within −4.7 to −6.9 kcal/mol) with specific HLA alleles. The epitope cluster is predicted to provide 93.86% population coverage worldwide. Based on our immunoinformatics analysis, we propose the peptide WLEARMLLDNIYLQDGLIASLYR as a new vaccine candidate against Zika virus for further validation.

    Original languageEnglish
    Article number100434
    Pages (from-to)1-12
    Number of pages12
    JournalInformatics in Medicine Unlocked
    Volume21
    DOIs
    Publication statusPublished - 2020

    Bibliographical note

    Copyright the Author(s) 2020. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

    Keywords

    • Epitope
    • Immunoinformatics
    • Vaccine
    • Zika virus
    • Proteome

    Fingerprint

    Dive into the research topics of 'Zika viral proteome analysis reveals an epitope cluster within NS3 helicase as a potential vaccine candidate: an in silico study'. Together they form a unique fingerprint.

    Cite this