Zinc uptake, oxidative stress and the FNR-like proteins of Lactococcus lactis

Colin Scott, Helen Rawsthorne, Manisha Upadhyay, Claire A. Shearman, Michael J. Gasson, John R. Guest, Jeffrey Green*

*Corresponding author for this work

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Lactococcus lactis ssp. cremoris MG1363 contains two FNR homologues, FlpA and FlpB, encoded by the distal genes of two paralogous operons (orfXA/B-orfYA/B-flpA/B). An flpA flpB double mutant strain is hypersensitive to hydrogen peroxide and has a depleted intracellular Zn(II) pool. The phenotypes of the flp mutant strains suggest that FlpA and FlpB control the expression of high and low affinity ATP-dependent Zn(II) uptake systems, respectively. Plate tests revealed that expression from a orfXB::lac reporter was activated by Cd(II), consistent with other Zn(II)-regulated systems. The link between a failure to acquire Zn(II) and hypersensitivity to oxidative stress suggests that Zn(II) may be required to protect vulnerable protein thiols from oxidation.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalFEMS Microbiology Letters
Volume192
Issue number1
DOIs
Publication statusPublished - 1 Nov 2000
Externally publishedYes

Keywords

  • lactic acid bacterium
  • zinc
  • oxidative stress
  • FNR-like protein
  • cadmium
  • thiol protection

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    Scott, C., Rawsthorne, H., Upadhyay, M., Shearman, C. A., Gasson, M. J., Guest, J. R., & Green, J. (2000). Zinc uptake, oxidative stress and the FNR-like proteins of Lactococcus lactis. FEMS Microbiology Letters, 192(1), 85-89. https://doi.org/10.1016/S0378-1097(00)00413-4