Zinc uptake, oxidative stress and the FNR-like proteins of Lactococcus lactis

Colin Scott; Helen Rawsthorne; Manisha Upadhyay; Claire A. Shearman; Michael J. Gasson; John R. Guest; Jeffrey Green

doi:10.1016/S0378-1097(00)00413-4

Zinc uptake, oxidative stress and the FNR-like proteins of Lactococcus lactis

Colin Scott, Helen Rawsthorne, Manisha Upadhyay, Claire A. Shearman, Michael J. Gasson, John R. Guest, Jeffrey Green^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Lactococcus lactis ssp. cremoris MG1363 contains two FNR homologues, FlpA and FlpB, encoded by the distal genes of two paralogous operons (orfX_A/B-orfY_A/B-flpA/B). An flpA flpB double mutant strain is hypersensitive to hydrogen peroxide and has a depleted intracellular Zn(II) pool. The phenotypes of the flp mutant strains suggest that FlpA and FlpB control the expression of high and low affinity ATP-dependent Zn(II) uptake systems, respectively. Plate tests revealed that expression from a orfX_B::lac reporter was activated by Cd(II), consistent with other Zn(II)-regulated systems. The link between a failure to acquire Zn(II) and hypersensitivity to oxidative stress suggests that Zn(II) may be required to protect vulnerable protein thiols from oxidation.

Original language	English
Pages (from-to)	85-89
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	192
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(00)00413-4
Publication status	Published - 1 Nov 2000
Externally published	Yes

Keywords

lactic acid bacterium
zinc
oxidative stress
FNR-like protein
cadmium
thiol protection

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10.1016/S0378-1097(00)00413-4

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title = "Zinc uptake, oxidative stress and the FNR-like proteins of Lactococcus lactis",

abstract = "Lactococcus lactis ssp. cremoris MG1363 contains two FNR homologues, FlpA and FlpB, encoded by the distal genes of two paralogous operons (orfXA/B-orfYA/B-flpA/B). An flpA flpB double mutant strain is hypersensitive to hydrogen peroxide and has a depleted intracellular Zn(II) pool. The phenotypes of the flp mutant strains suggest that FlpA and FlpB control the expression of high and low affinity ATP-dependent Zn(II) uptake systems, respectively. Plate tests revealed that expression from a orfXB::lac reporter was activated by Cd(II), consistent with other Zn(II)-regulated systems. The link between a failure to acquire Zn(II) and hypersensitivity to oxidative stress suggests that Zn(II) may be required to protect vulnerable protein thiols from oxidation.",

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author = "Colin Scott and Helen Rawsthorne and Manisha Upadhyay and Shearman, {Claire A.} and Gasson, {Michael J.} and Guest, {John R.} and Jeffrey Green",

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T1 - Zinc uptake, oxidative stress and the FNR-like proteins of Lactococcus lactis

AU - Scott, Colin

AU - Rawsthorne, Helen

AU - Upadhyay, Manisha

AU - Shearman, Claire A.

AU - Gasson, Michael J.

AU - Guest, John R.

AU - Green, Jeffrey

PY - 2000/11/1

Y1 - 2000/11/1

N2 - Lactococcus lactis ssp. cremoris MG1363 contains two FNR homologues, FlpA and FlpB, encoded by the distal genes of two paralogous operons (orfXA/B-orfYA/B-flpA/B). An flpA flpB double mutant strain is hypersensitive to hydrogen peroxide and has a depleted intracellular Zn(II) pool. The phenotypes of the flp mutant strains suggest that FlpA and FlpB control the expression of high and low affinity ATP-dependent Zn(II) uptake systems, respectively. Plate tests revealed that expression from a orfXB::lac reporter was activated by Cd(II), consistent with other Zn(II)-regulated systems. The link between a failure to acquire Zn(II) and hypersensitivity to oxidative stress suggests that Zn(II) may be required to protect vulnerable protein thiols from oxidation.

AB - Lactococcus lactis ssp. cremoris MG1363 contains two FNR homologues, FlpA and FlpB, encoded by the distal genes of two paralogous operons (orfXA/B-orfYA/B-flpA/B). An flpA flpB double mutant strain is hypersensitive to hydrogen peroxide and has a depleted intracellular Zn(II) pool. The phenotypes of the flp mutant strains suggest that FlpA and FlpB control the expression of high and low affinity ATP-dependent Zn(II) uptake systems, respectively. Plate tests revealed that expression from a orfXB::lac reporter was activated by Cd(II), consistent with other Zn(II)-regulated systems. The link between a failure to acquire Zn(II) and hypersensitivity to oxidative stress suggests that Zn(II) may be required to protect vulnerable protein thiols from oxidation.

KW - lactic acid bacterium

KW - zinc

KW - oxidative stress

KW - FNR-like protein

KW - cadmium

KW - thiol protection

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DO - 10.1016/S0378-1097(00)00413-4

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AN - SCOPUS:0034333354

SN - 0378-1097

VL - 192

SP - 85

EP - 89

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Zinc uptake, oxidative stress and the FNR-like proteins of Lactococcus lactis

Abstract

Keywords

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